SDF-1beta/CXCL12, feline recombinant | 4389

Stromal Derived Growth Factor-1beta (SDF-1beta), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1 signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human SDF-1 shares 100% identity to feline SDF-1. Recombinant feline SDF-1beta is a non-glycosylated protein, comprised of 72 amino acids, with a molecular weight of 8.5 kDa.

4389 | SDF-1beta/CXCL12 feline recombinant DataSheet

Biomolecule/Target: SDF-1 beta

Synonyms: SDF1

Alternates names: CKTSF1B1, DAND2, DRM, IHG-2

Taglines: A small cytokine strongly chemotactic for lymphocytes

NCBI Gene ID #: 23892

NCBI Gene Symbol: Grem1

Gene Source: Human

Accession #: O70326

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11 g/ml

Biological activity: Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.3 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: KKKGSQGAIP PPDKAQHNDS EQTQSPQQPG SRNRGRGQGR GTAMPGEEVL ESSQEALHVT ERKYLKRDWC KTQPLKQTIH EEGCNSRTII NRFCYGQCNS FYIPRHIRKE EGSFQSCSFC KPKKFTTMMV TLNCPELQPP TKKKRVTRVK QCRCISIDLD