451 Recombinant Proteins and Cell culture

Sec21p | Gamma subunit, COP vesicles | AS08 327

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451-AS08 327
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Description

Sec21p | Gamma subunit, COP vesicles | AS08 327 | Gentaur UK, US & Europe Distribution

Immunogen: GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunofluorescence (IF), Western blot (WB)

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Recommended Dilutions: 1 : 1000 (IF), 1 : 1000 (WB)

Molecular weight: 98 kDa

Confirmed Reactivity: Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays

Predicted Reactivity: Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays, Species of your interest not listed? Contact us

Not reactive in: Nicotiana tabacum, Microsporidia sp.

Additional Information: This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000) . In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12 (11) :2219-36. Ritzenthaler et al. (2002) . Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14 (1) :237-61.

Background: Sec21p is a constituent of the COPI vesicle coatomer.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G34450

Category: Endomembrane system

Research Area: Cell Biology

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Additional Information

Size:
50 µL
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