SEMA3E Antibody | 46-347

SEMA3E Antibody | 46-347 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: N/A

Immunogen: The immunogen for this antibody is: C-KPEHYRLPRHTLDS

Research Area: Cancer

Tested Application: E, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 35kDa and 20kDa in Human Prostate lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 89.2kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. XBL-11050 - Human Adrenal Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: SEMA3E, SEMAH, coll-5, M-SEMAH, M-SemaK, KIAA0331, M-sema H, sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3E, sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, 3E

User Note: N/A

BACKGROUND: N/A