Description
Sepadextran-25 Fine - Cat Number: SD25F. Supplier: .
Description:
Sepadextran™ is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Sepadextran™-25 can be used for protein and nucleic acid purifications being the exclusion limit 5 kD for proteins and 10 bases for nucleic acids. Desalting (before IEX and after HIC or Affinity Chromatography) & buffer exchange (between different chromatography steps) are other uses.
Fine grade are for preparative separations and routine laboratory work.
Fine grade are for preparative separations and routine laboratory work.
Product Data:
| Product Name | Sepadextran™-25 Fine |
| Cat. No. | SD25F-X |
| Matrix | Crosslinked dextran |
| Water Regain | 2.15-2.25 ml/g |
| Swelling | 4-6 ml/g |
| Dry Particle Size | 20-80 µm (>80%) |
| Wet Particle Size | 35-140 µm |
| Maximum Operating Pressure | Generally obeys Darcy’s Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm). Ko=30 |
| Fractionation Range | 1-5 kD for globular proteins, 100-5,000 Da for dextrans |
| pH Stability | 2.0 to 13 |
| Autoclavable | 121˚C, pH 7.0 (30 minutes) |
| Chemical Stability | All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile |
| Remarks | This product is produced based on a customer order. Delivery time may be longer. |
| Storage Temperature | Ambient |
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