Description
Serotonin receptor 3A Antibody | 46-357 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Rat
Homology: N/A
Immunogen: The immunogen for this antibody is: C-GPQDFEKSPRDR
Research Area: Cancer
Tested Application: E, WB, IHC, ICC, IF
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.2. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml. ICC/ IF:This product has been successfully used in IF/ ICC on Rat: Lu CL et al, Gastroenterology. 2009 Sep;137 (3) :1040-50. PMID: 19344720.
Specificiy: This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1) .
Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: HTR3A, serotonin receptor, 5-hydroxytryptamine (serotonin) receptor 3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine (serotonin) receptor-3, 5HT3 serotonin receptor, Serotonin-gated ion channel receptor, truncated receptor, containing only 3 transmembrane domains
User Note: N/A
BACKGROUND: N/A