SF-20 Antibody | 38-189

SF-20 Antibody | 38-189 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography employing immobilized mSF-20 matrix.

Research Area: Antibody Pairs

Tested Application: E, WB

Application: ELISA:
Indirect:
To detect mSF-20 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.
Sandwich
To detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine SF-20 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Western Blot To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: SF-20 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: Il25, Ly6elg, Il25, UPF0556 protein C19orf10 homolog, Interleukin-25, IL-25

User Note: Centrifuge vial prior to opening.

BACKGROUND: SF20, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown