SHBG Antibody | 7935

SHBG Antibody | 7935 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (75%)

Immunogen: SHBG antibody was raised against a 16 amino acid peptide near the center of human SHBG.
The immunogen is located within amino acids 220 - 270 of SHBG.

Research Area: Obesity

Tested Application: E, WB, IF

Application: SHBG antibody can be used for detection of SHBG by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: SHBG antibody is human, mouse, and rat reactive. At least three isoforms of SHBG are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 25, 26, 31, 32, 38, 44 kDa
Observed: 35 kDa

Validation: N/A

Isoform: N/A

Purification: SHBG antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SHBG antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SHBG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Sex hormone-binding globulin, ABP, SBP, TEBG

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SHBG is a steroid binding protein that was first described as a plasma protein secreted by the liver and is thought to participate in the regulation of steroid responses. SHBG transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers (1) . Low plasma SHBG levels are associated with obesity, abdominal adiposity, metabolic syndrome, and predict the development of type 2 diabetes (2, 3) . Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus (4) .