Sheep GPX2 (Glutathione Peroxidase 2, Gastrointestinal) ELISA Kit | ELK8722

Sheep GPX2 (Glutathione Peroxidase 2, Gastrointestinal) ELISA Kit | ELK8722 | Gentaur UK, US & Europe Distribution

Alternative Names: GI-GPx; GPRP; GSHPX-GI; GSHPx-2; Gastrointestinal glutathione peroxidase; Glutathione peroxidase-gastrointestinal; Glutathione peroxidase-related protein 2

Species: Sheep

Assay Type: Sandwich

Sensitivity: 0.127 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 2, Gastrointestinal (GPX2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 2, Gastrointestinal (GPX2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 2, Gastrointestinal (GPX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 2, Gastrointestinal (GPX2) in the samples is then determined by comparing the OD of the samples to the standard curve.