SIGLEC7 Antibody (D-siglec)| F40103-0.08ML

SIGLEC7 Antibody (D-siglec)| F40103-0.08ML | Gentaur UK, US & Europe Distribution

Background: SIGLECs are cell surface proteins of the Ig superfamily. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. A large subgroup of SIGLECs share high homology with SIGLEC3 (CD33) and are localized to 19q13.4. The cDNA for the SLG gene encodes 2 variants, SLG-long (SLGL) and SLG-short (SLGS). The 595-amino acid SLGL protein contains a signal peptide and 2 V-set N-terminal Ig-like domains. The 477-amino acid SLGS protein has a weak signal sequence and, like most SIGLEC3-like SIGLECs, has only 1 V-set N-terminal Ig-like domain. Both variants contain 2 C2-set N-terminal Ig-like domains, a transmembrane domain, and a cytoplasmic tail with a putative ITIM and a putative SLAM-like tyrosine-based motif. The conserved arginine residue thought to be essential for sialic acid binding in other SIGLECs is replaced by a glutamine in SLGS and by a cysteine in SLGL. RT-PCR analysis detected high expression of both variants in spleen and small intestine, and SLGS was highly expressed in adrenal gland and SLGL was highly expressed in bone marrow.

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: N/A

Application: WB, IHC, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100

Application Note: Titration of the SIGLEC7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This SIGLEC7 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this SIGLEC7 antibody.

Storage: Aliquot the SIGLEC7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A