SIRT1 Antibody | 42-428

SIRT1 Antibody | 42-428 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse

Immunogen: The immunogen for this antibody is: C-EKPQEVQTSRNVES

Research Area: Other

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100 (19) :10794-9.; PMID: 12960381) . Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5ug/ml.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1) .

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: SIRT1, sirtuin 1, SIR2L1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, sir2-like 1, sirtuin type 1

User Note: N/A

BACKGROUND: N/A