SIRT1 polyclonal Antibody | BS64501

SIRT1 polyclonal Antibody | BS64501 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB

Application Range: WB: 1:2000~1:5000

Background: The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD) -dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein (8) . Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SIRT1 polyclonal Antibody detects endogenous levels of SIRT1 protein.

Molecular Weight: ~120kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: NAD-dependent protein deacetylase sirtuin-1;hSIRT1;NAD-dependent protein deacylase sirtuin-1;Regulatory protein SIR2 homolog 1;SIR2-like protein 1; hSIR2;SirtT1 75 kDa fragment;75SirT1;SIRT1;SIR2L1

Immunogen: Synthetic peptide, corresponding to Human SIRT1.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: