SIRT5 (GST-tagged), Human recombinant | 7674

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-7. SIRT5 is located in the mitochondrial matrix and its functions are larGely still being elucidated, however a few promising substrates have been studied. SIRT5 has been shown to deacetylate carbamoyl phosphate synthetase 1 (CSP1), activating the enzyme to catalyze the first step of the urea cycle. CSP1 is important in the detoxification of excess ammonia that can accumulate during fasting. Cytochrome C, another mitochondrial enzyme involved in oxidative metabolism can also be deacetylated by SIRT5. SIRT5 is predominantly found in the heart, brain, testis, and lymphoblasts.

7674 | SIRT5 GST-tagged Human recombinant DataSheet

Biomolecule/Target: SIRT5

Synonyms: SIRT5 (GST-tagged), Human recombinant

Alternates names: IFN alpha II-1, IFNW1

Taglines: Intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

NCBI Gene ID #: 338376

NCBI Gene Symbol: IFNE1

Gene Source: Human

Accession #: Q86WN2

Recombinant: Yes

Source: E Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): The ED50 was determined by a cytotoxicity assay using Human TF-1 cells is 0.01 ng/ml, corresponding to a specific activity of 1 x 10^8 units/mg.

Biological activity: The ED50 was determined by a cytotoxicity assay using Human TF-1 cells is 0.01 ng/ml, corresponding to a specific activity of 1 x 10^8 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS