SIRT7 Antibody | 6107

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. SIRT7 is a member of this family of proteins and is present in prokaryotes and eukaryotes. All SIR2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast, and mammalian sirtuins are able to metabolize NAD+ and several act as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but as mentioned above, recent reports of histone-activated SIR2-mediated NAD+ metabolism and NAD+-activated SIR2-mediated histone deacetylation s?ggest a possible coupled reciprocal activation mechanism involving interactions of SIR2 with NAD+ and the N-?-acetyl-lysine groups of acetylated histone.

6107 | SIRT7 Antibody DataSheet

Antibody Target: SIRT7

Target Alternative Name: SIR2; Sirtuin 7; Silent Information Regulator 7

Tag Line: Polyclonal antibody to detect SIRT7 (a lysyl-deacetylase) in human samples

Category: Epigenetics

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: Human SIRT7 aa 35-51 and 361-377

Accession #: Q9NRC8

Gene ID: 51547

Appearance: Colorless liquid

Form: Liquid

Concentration: N/A

Formulation: 50 µg of antibody in 100 µl PBS containing 0.2% gelatin and 0.05% sodium azide

Purification: Protein G purified

Application: Western blot

Positive Control: Western blot: Human liver homogenate, PBMC lysate

Application And Usages: Western blot: 1-3 µg/ml. However, the optimal conditions should be determined individually.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified