Smac Antibody | 2411

Smac Antibody | 2411 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-Smac antibody (2411) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of murine Smac.
The immunogen is located within the last 50 amino acids of Smac.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF, IP

Application: WB: 1 μg/mL; IF: 10 μg/mL; IHC: 2 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1401 - Mouse Heart Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 27kD
Observed: 20 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar Smac expression profile in human and mouse cell lines detected by two independent anti-Smac antibodies that recognize different epitopes, 2409 against human C-terminus domain and 2411 against murine C-terminus domain.  Smac proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

KO Validation (Figure 6) : Anti-Smac antibody specificity was further verified by Smac knockout mice. Smac signal smac KO mice was disrupted as compared to control.

Induced Expression Validation (Figure 6, 9) : Smac expression detected by anit-Smac antibodies was up-regulated by STS or rPVL treatment.

Overexpression Validation (Figure 10) : Smac overexpression was detected by anit-Smac antibodies on smac transfected HEK293T cells.

Isoform: Human Smac has 3 isoforms, including isoform 1 (239aa, 27.1kD) , isoform 2 (186aa, 21.2kD) , and isoform 3 (195aa, 22.3kD) . Mouse Smac has one isoform (237aa, 26.8kD) and Rat Smac also has one isoform (237aa, 26.9kD) . 2411 can detect human, mouse and rat.

Purification: Smac Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Smac Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Smac antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Smac Antibody: Smac, AU040403, 0610041G12Rik, 1700006L01Rik, Smac, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.