SMT1 | Sterol methyltransferase 1 | AS07 266

SMT1 | Sterol methyltransferase 1 | AS07 266 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 UniProt: Q9LM02, TAIR: At5g13710

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunolocalization (IL), Western blot (WB)

Related Products: AS09 481 | Anti-BiP2 | luminal-binding protein 2, rabbit antibodiesAntibodies to other proteins of endomembrane system

Recommended Dilutions: 1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)

Molecular weight: 38 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Amborella trichopoda, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in: Hordeum vulgare, Triticum aestivum, Withania somnifera

Additional Information: SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010) . Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Background: SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G13710

Category: Endomembrane system

Research Area: Cell Biology