SMURF1 Antibody | 7945

SMURF1 Antibody | 7945 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1.
The immunogen is located within amino acids 180 - 230 of SMURF1.

Research Area: Signal Transduction, Cancer

Tested Application: E, WB, IHC-P

Application: SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Specificiy: SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 80, 83 kDa
Observed: 78 kDa

Validation: N/A

Isoform: N/A

Purification: SMURF1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SMURF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SMAD specific E3 ubiquitin protein ligase 1, SMAD ubiquitination regulatory factor 1, E3 ubiquitin-protein ligase SMURF1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1) . SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1, 2) . SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3) . SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4) .