SOX2 Antibody | 5583

SOX2 Antibody | 5583 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Sheep: (100%) , Chicken: (100%) , Horse: (83%) , Pig: (100%)

Immunogen: SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX.
The immunogen is located within the first 50 amino acids of SOX2.

Research Area: Homeostasis, Stem Cell

Tested Application: E, WB, ICC, IHC-P, IF

Application: SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: SOX2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SOX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SOX2 Antibody: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.