SPIB Antibody | 8157

SPIB Antibody | 8157 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (88%)

Immunogen: SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB.
The immunogen is located within amino acids 200 - 250 of SPIB.

Research Area: Stem Cell, Immunology

Tested Application: E, WB, IHC-P, IF

Application: SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

Positive Control 1: Cat. No. 1312 - Human Prostate Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 19, 25, 29 kDa
Observed: 20 kDa

Validation: N/A

Isoform: N/A

Purification: SPIB antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SPIB antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SPIB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Spi-B transcription factor, SPI-B

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1) . SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2, 3) . It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN) -producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3, 4) .