SPN/CD43 Antibody (N-term) [AMM07960G]

SPN/CD43 Antibody (N-term) [AMM07960G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SPN/CD43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human SPN/CD43.

Cellular Localisation: Membrane; Single-pass type I membrane protein. Cell projection, microvillus {ECO:0000250|UniProtKB:P13838}. Cell projection, uropodium {ECO:0000250|UniProtKB:P15702}. Note=Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN) {ECO:0000250|UniProtKB:P13838, ECO:0000250|UniProtKB:P15702}

Molecular Weight: 40322

Clone: Polyclonal

Gene Name: SPN

Gene ID: 6693

Function: Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity) . Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4 (+) T-cells and to a lesser extent by CD8 (+) T-cells (PubMed:18036228) . Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034) . Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E- selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity) .

Summary: Tissue Location: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, SPN, CD43