SPP1 Antibody | 7443

SPP1 Antibody | 7443 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (94%) , Bovine: (83%)

Immunogen: SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1.
The immunogen is located within amino acids 50 - 100 of SPP1.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.

Positive Control 1: Cat. No. 1310 - Human Bladder Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 36 kDa
Observed: 37 kDa

Validation: N/A

Isoform: N/A

Purification: SPP1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SPP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SPP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SPP1 Antibody: OPN, BNSP, BSPI, ETA-1, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, SPP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SPP1 Antibody: The secreted protein 1 (SPP1) , also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1, 2) . SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2) . SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4) .