SPPL3 Antibody (N-term) [APR05741G]

SPPL3 Antibody (N-term) [APR05741G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SPPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-81 amino acids from the N-terminal region of human SPPL3.

Cellular Localisation: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus. Membrane; Multi-pass membrane protein; Lumenal side

Molecular Weight: 42261

Clone: Polyclonal

Gene Name: SPPL3 {ECO:0000303|PubMed:12077416, ECO:0000312|HGNC:HGNC:30424}

Gene ID: 121665

Function: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries (PubMed:16873890, PubMed:25354954, PubMed:25827571) . Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571) . Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC) -mediated cleavage proteolysis (PubMed:23132852) . May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130 (PubMed:23132852) . Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954) . Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease- independent manner (PubMed:25384971) .

Summary: Tissue Location: Widely expressed (PubMed:15385547) . Expressed in the brain (PubMed:11978763) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Signal peptide peptidase-like 3, SPP-like 3, 3423-, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4, SPPL3, IMP2, PSL4