Spred3 Antibody | 4851

Spred3 Antibody | 4851 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.
The immunogen is located within amino acids 140 - 190 of Spred3.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 45 kDa
Observed: 45 kDa

Validation: N/A

Isoform: N/A

Purification: Spred3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Spred3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3, Spred-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD) . The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.