SQSTM1 Antibody | 5449

SQSTM1 Antibody | 5449 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: SQSTM1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1.
The immunogen is located within the last 50 amino acids of SQSTM1.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-2 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL;.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 2: Cat. No. 1204 - K562 Cell Lysate

Positive Control 3: Cat. No. 1201 – HeLa Cell Lysate

Positive Control 4: Cat. No. 1203 – A549 Cell Lysate

Positive Control 5: Cat. No. 1282 – 3T3/NIH Cell Lysate

Positive Control 6: N/A

Molecular Weight: Predicted: 48kD
Observed: 65 kD

Validation:

KO Validation (Figure 1) : Anti-SQSTM1 antibodies (5449) specificity was further verified by SQSTM1 specific knockout. SQSTM1 signal was not detected in SQSTM1 knockout HEK293T cells as compared to that in control wild type cells.

Induced Expression Validation (Figure 2) : Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (0-24 hrs) . SQSTM1 expression detected by anit-SQSTM1 antibodies (5449) was induced by LPS treatment and the expression levels were increased gradually over the period of 24 hrs.

Isoform: Human SQSTM1 has two isoforms, including isoform 1 (440aa, 47.7kD) and isoform 2 (356aa, 38.6kD) . Mouse SQSTM1 also has two isoforms, including isoform 1 (442aa, 48.2kD) and isoform 2 (404aa, 44.2kD) . Rat SQSTM1 has three isoforms, including isoform 1 (439aa, 47.7kD) , isoform 2 (412aa, 45kD) and isoform 3 (234aa, 26.1kD) . 5449 can detect the isoforms of human mouse and rat except the shortest rat isoform 3.

Purification: SQSTM1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SQSTM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SQSTM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SQSTM1 Antibody: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-κB by TNF-α, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.