SSA ELISA kit | 55R-ORG652

SSA ELISA kit | 55R-ORG652 |

Activity Code: ACTIVE

Product Type: Research Kits

Product Subtype: Kits

Research Area: DNA & RNA

Short Description: ELISA kit for the detection of SSA in the research laboratory

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: N/A

Storage: Store at 2-8 deg C

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end-user

Assay Information: AntiSSA 52 is an indirect solid phase enzyme immunometric assay (ELISA) . It is designed for the quantitative measurement of IgG class autoantibodies directed against the extractable nuclear antigens SSA 52 (Ro 52) . The microplate is coated with SSA 52 (Ro 52) . The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway") . The binding of  present autoantibodies, as well as the formation of the sandwich complexes and enzymatic color reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended. The optical density for each calibrator may be graphically plotted against the concentration of IgG and unknowns extrapolated from the curve.