SSA IgG (Human) ELISA Kit - E4979

E4979 | SSA IgG (Human) ELISA Kit DataSheet

Sjogren syndrome (SS)A/Ro are autoantigens that are a type of extractable nuclear antigen (ENA). SSA consists of 2 proteins: Ro60 and Ro52. Ro60 binds to uncoded RNA to form an hY-RNA complex and thereby inhibits the immune response. Ro52 is a protein formed either due to viral infection, type I interferons, or Toll-like receptors. Antibodies to SSA/Ro antigen are detected in patients suffering from autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren's syndrome (SS), systemic sclerosis, and rheumatoid arthritis (RA). ’s SSA IgG (Human) ELISA kit quantitatively measures SSA IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with SSA antigen. IgG antibodies to SSA, if present in the samples, will bind to the SSA antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to SSA present in the sample

Alternate Name : anti-SSA, SSA (Ro), SSA, anti-SS-A, anti-SS-A-Ro, Sjogren's anti SS-A, Sjogren's anti SSA

Tag Line : An ELISA kit for quantitative measurement of SSA IgG in human serum and plasma samples

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma Serum

Species Reactivity : Human

Applications :

Features and Benefits : Easy, convenient and time-saving method to measure the amount of SSA IgG in serum and plasma samples No significant cross-reactivity or interference with other autoantibodies was observed