ST6GalNAc1, soluble fragment, Human Recombinant | P1223

Alpha-N-acetylgalactosaminide -2,6-sialyltransferase 1 catalyzes the transfer of sialic acid N-acetylneuraminic acid (Neu5Ac) from cytidine 5'-monophosphono-N-acetylneuraminic acid (CMP-Neu5Ac) to O-linked GalNAc residues in an -2,6 linkage. ST6GalNAcI belongs to a large family of Golgi-membrane-bound sialyltransferases that is responsible for the biosynthesis of sialyated structures, and from which approximately 20 have been cloned to date. The cancer associated sialyl-Tn (sTn) angtigen is formed by ST6GalNAcI, through the transfer of Neu5Ac to GalNAc resiudes on mucins. Addition of a sialic acid to a glyco-protein effectively caps the glycan-chain, preventing further additions. The presence of sialic acid residues extends the half-life of circulating proteins, and are considered to be important for a large variety of biotherapuetics.

P1223 | ST6GalNAc1 soluble fragment Human Recombinant DataSheet

Biomolecule/Target: ST6GALNAC1

Synonyms: Beta-1,4-galactosyltransferase 1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, Beta-1,4-GalTase 1, Beta4Gal-T1

Alternates names: Beta-1,4-galactosyltransferase 1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, Beta-1,4-GalTase 1, Beta4Gal-T1

Taglines: catalyzes the transfer of sialic acid N-acetylneuraminic acid

NCBI Gene ID #: 28106

NCBI Gene Symbol: CS010

Gene Source: Human

Accession #: Q9CPT4

Recombinant: Yes

Source: Insect cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by transfer of NeuAc from CMP-NeuAc to the Tn-peptide MEELGMAPALQPT(GalNAc)QGAMPAF.

Biological activity: N/A

Results: > 50 pmol/min/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 50- 60 kDa

Concentration: 500 ug/ml

Appearance: Liquid

Physical form description: Sterile filtered solution in 20 mM Hepes pH7 and 100 mM NaCl

Reconstitution Instructions: Reconstitute in sterile ddHO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL