STAT1 alpha Antibody | 1021

STAT1 alpha Antibody | 1021 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (94%)

Immunogen: Anti-STAT1 alpha antibody (1021) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha.
The immunogen is located within the last 50 amino acids of STAT1 alpha.

Research Area: Signal Transduction

Tested Application: E, WB, ICC, IP, IHC-P, IF

Application: WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2.5 μg/mL; ICC: 10 μg/mL .
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence, Immunohistochemistry and Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 1202 - A431 Cell Lysate

Positive Control 4: Cat. No. 1204 - K562 Cell Lysate

Positive Control 5: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 6: N/A

Molecular Weight: Predicted: 87kD
Observed: 87 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar STAT1 alpha expression profile in human cell lines detected by two independent anti-STAT1 alpha  antibodies that recognize different epitopes, 1021 against C-terminus domain and competitor antibody  against C-terminus domain. STAT1 alpha proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human STAT1 alpha has two isoforms, including isoform 1 (750aa, 87.3kD) and isoform 2 (712aa, 83kD). Mouse STAT1 alpha has one isoform (749aa, 87.2kD) and Rat STAT1 alpha also has one isoform (749aa, 87.2kD). 1021 can detect longer human isoform, mouse and rat, but it cannot detect the shorter human isoform.

Purification: STAT1 alpha Antibody is Antibody is DEAE purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: STAT1 alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: STAT1 alpha Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-α, IFN-β, IFN-γ and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1).