STIM1 Antibody | 4119

STIM1 Antibody | 4119 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.
The immunogen is located within the last 50 amino acids of STIM1.

Research Area: Immunology, Cancer

Tested Application: E, WB, IHC-P, IF

Application: STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.

Positive Control 1: Cat. No. 1409 - Mouse Thymus Tissue Lysate

Positive Control 2: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 3: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 59, 75, 87 kDa
Observed: 88 kDa

Validation: N/A

Isoform: N/A

Purification: STIM1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: STIM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, Stromal interaction molecule 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: STIM1 Antibody: In T lymphocytes, the sole pathway for Ca²⁺ entry following antigen-receptor binding is through store-operated Ca²⁺-release-activated Ca²⁺ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1) , a protein that functions as a Ca²⁺ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER) -like sites which act as the Ca²⁺ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca²⁺ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca²⁺ entry.