STIM1 Antibody (C-term) [APR10301G]

STIM1 Antibody (C-term) [APR10301G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This STIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 509-538 amino acids from the C-terminal region of human STIM1.

Cellular Localisation: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single- pass type I membrane protein. Cytoplasm, cytoskeleton. Sarcoplasmic reticulum. Note=Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane (PubMed:19249086, PubMed:16005298, PubMed:16208375, PubMed:18854159) Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184) . Colocalizes with ORAI1 at the cell membrane (PubMed:27185316) . Colocalizes preferentially with CASQ1 at endoplasmic reticulum in response to a depletion of intracellular calcium (PubMed:27185316) .

Molecular Weight: 77423

Clone: Polyclonal

Gene Name: STIM1

Gene ID: 6786

Function: Plays a role in mediating store-operated Ca (2+) entry (SOCE), a Ca (2+) influx following depletion of intracellular Ca (2+) stores (PubMed:15866891, PubMed:16005298, PubMed:16208375, PubMed:16537481, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:18854159, PubMed:19249086, PubMed:22464749, PubMed:24069340, PubMed:24351972, PubMed:24591628, PubMed:26322679, PubMed:25326555, PubMed:28219928) . Acts as Ca (2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca (2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca (2+) release- activated Ca (2+) (CRAC) channel subunit ORAI1 (PubMed:16208375, PubMed:16537481) . Involved in enamel formation (PubMed:24621671) . Activated following interaction with STIMATE, leading to promote STIM1 conformational switch (PubMed:26322679) .

Summary: Tissue Location: Ubiquitously expressed in various human primary cells and tumor cell lines.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Stromal interaction molecule 1, STIM1, GOK