SUB1 polyclonal Antibody | BS8687

SUB1 polyclonal Antibody | BS8687 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse

Application: WB,IHC,IF

Application Range: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: RNA polymerase II (RNAPII) holoenzyme contains numerous proteins that largely consist of RNA processing factors, RNA helicase, general transcription factors and SRB co-activating complexes. RNAPII mediated basal- and gene-specific transcriptional activation requires the association of various cofactors that includes PC4 (human Positive cofactor 4) . Positive cofactor 4 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP) -associated factors (TAFs) to directly bind to double stranded DNA. Positive cofactor 4 induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Addition-ally, Positive cofactor 4 is phosphorylated by TFIID and TFIIH, which releases Positive cofactor 4 from the DNA promoter region and thereby inhibits the assembly of Positive cofactor 4 into the transcriptional promoting complex and blocks transcription.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SUB1 polyclonal Antibody detects endogenous levels of SUB1 protein.

Molecular Weight: ~ 14kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; PC4; SUB1 homolog; p14; SUB1; PC4, RPO2TC1

Immunogen: Recombinant full length Human SUB1.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: