SUMO1 | Small ubiquitin-like modifier protein 1 | AS08 308

SUMO1 | Small ubiquitin-like modifier protein 1 | AS08 308 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein G purified in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS08 307A | Anti-UBQ11 | Ubiquitin, rabbit antibody (affinity purified) AS08 307 | Anti-UBQ11 | Ubiquitin, rabbit antibody, (serum) AS08 349 | Anti-SUMO3 | Small ubiquitin-like modifier protein 3, rabbit antibodiesPlant protein extraction buffer

Recommended Dilutions: 1 : 1000-1 : 5000 (WB)

Molecular weight: 10, 97 | 12 kDa

Confirmed Reactivity: Arabidopsis thaliana, Oryza sativa, Solanum tuberosum

Predicted Reactivity: Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea maysSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Antibodies will also detect SUMO2 protein. Suggested extraction buffer: 100mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50mM sodium metabisulfite, 20mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018) . This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.

Background: SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G26840

Category: Signal transduction

Research Area: Cell Signaling