SUPT5H polyclonal Antibody | BS71808

SUPT5H polyclonal Antibody | BS71808 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse

Application: WB

Application Range: WB 1:200 - 1:2000

Background: SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans. Nuclear SPT4 and SPT5 are involved in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) -mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat. SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation. However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation. The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SUPT5H polyclonal Antibody detects endogenous levels of SUPT5H protein.

Molecular Weight: 170kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: SPT5; SPT5H; Tat-CT1

Immunogen: Recombinant protein of human SUPT5H

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: