Survivin, human recombinant | 4160

Survivin, a member of the inhibitor of apoptosis (IAP) family, is also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5 and in humans is encoded by the BIRC5 gene. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis. Disruption of survivin induction pathways leads to an increase in apoptosis and decrease in tumour growth. Survivin is highly expressed in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells, making survivin an ideal target for studying cancer therapy.

4160 | Survivin human recombinant DataSheet

Biomolecule/Target: Survivin

Synonyms: BIRC5; API4; EPR-1

Alternates names: Macrophage Inflammatory Protein-1, CCL9/10, MRP2, CCF18

Taglines: An inhibitor of caspase activation/apoptosis

NCBI Gene ID #: 20308

NCBI Gene Symbol: CCL9

Gene Source: Murine

Accession #: P51670

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q