SUZ12 Antibody | 7701

SUZ12 Antibody | 7701 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (79%)

Immunogen: SUZ12 antibody was raised against a 19 amino acid peptide near the center of human SUZ12.
The immunogen is located within amino acids 400 - 450 of SUZ12.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SUZ12 antibody is human specific.

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 81 kDa
Observed: 96 kDa

Validation: N/A

Isoform: N/A

Purification: SUZ12 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SUZ12 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SUZ12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1) . SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2) . It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3, 4) . SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5) .