SWAP70 Antibody [AMM02405G]

SWAP70 Antibody [AMM02405G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: Purified His-tagged SWAP70 protein (Fragment) was used to produced this monoclonal antibody.

Cellular Localisation: Cytoplasm. Cell membrane. Nucleus. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Note=In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus (PubMed:10681448) In activated, class-switching B-cells it is associated with membrane IgG but not IgM (PubMed:10681448) . Localized to loose actin filament arrays located behind actively extending lamellipodia (PubMed:12925760) .

Molecular Weight: 68998

Clone: Monoclonal

Gene Name: SWAP70

Gene ID: 23075

Function: Phosphatidylinositol 3, 4, 5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3, 4) -bisphosphate production and cell protrusion (By similarity) .

Summary: Tissue Location: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448) . Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760) .

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:100~1000

Synonyms: Switch-associated protein 70, SWAP-70, SWAP70, KIAA0640