Symmetric DiMethyl-Histone H3-R8 pAb | 18-637

Symmetric DiMethyl-Histone H3-R8 pAb | 18-637 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat, Other

Homology: N/A

Immunogen: A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3

Research Area: Signal Transduction

Tested Application: WB, IHC, IF, IP, ChIP, ChIPseq

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
CHIP: 1:20 - 1:50
CHIP seq: 1:20 - 1:50

Specificiy: N/A

Positive Control 1: HeLa

Positive Control 2: H3 protein

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 18kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: H3F3A, H3t, H3.4, H3/g, H3FT

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.