TACE Antibody | 1131

TACE Antibody | 1131 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Rat

Homology: Predicted species reactivity based on immunogen sequence: mouse (94.1%)

Immunogen: Anti-TACE antibody (1131) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE.
The immunogen is located within the last 50 amino acids of TACE.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: WB: 0.25-2 μg/mL; ICC: 10 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples; and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Specificiy: 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 1207 - Raji Cell Lysate

Positive Control 4: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 93kD
Observed: 93-125kD (Post-modification: 9 N-linked glycosylation)

Validation:

KO Validation (Figure 2): Anti-TACE antibodies (1131) specificity was further verified by TACE specific knockout. TACE signal was not detected in TACE knockout HeLa cells as compared to that in control wild type cells.

Independent Antibody Validation in Cell lines (Figure 3) shows similar TACE expression profile in human cell lines detected by two independent anti-TACE antibodies that recognize different epitopes, 1131 against C-terminus domain and  22-001 against a synthetic peptide to a sequence within amino acids 700-824 of human TACE.  TACE proteins are detected in most of the tested cell lines except 293 and A549 cell lines at different expression levels by the two independent antibodies.  

KD validation (Figure 6,7): Anti-TACE antibody (1131) specificity was verified by TACE TACE specific siRNA or shRNA knockdown. TACE signal in COS cells (Figure 6) and MDA-MB-435 cells (Figure 7) with TACE knockdown was disrupted in comparison with control.

Overexpression validation (Figure 8): Anti-TACE antibody (1131) detected high expression levels of TACE in MCF-7 cells transfected with TACE as compared to vector transfected cells.

Isoform: Human TACE has 2 isoforms, including isoform A (824aa, 93kD), isoform B (694aa, 78.5kD). This antibody detects only isoform A. Mouse TACE has 2 isoforms, long isoform (827aa, 93.1kD) and short isoform (655aa, 73.9kD). Rat TACE has only one isoform identified so far (827aa, 93kD).

Purification: TACE Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TACE Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TACE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TACE Antibody: CSVP, TACE, NISBD, ADAM18, CD156B, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, ADAM 17

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.