TAF1 bromodomain 1 (1371-1496 aa) (GST-tagged), Human recombinant | 7660

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. TAF1 is a component of transcription factor IID, and binds to core promoter sequences at the transcription start site. TAF1 helps control transcription by both its kinase and histone acetyltransferase enzymatic activities. It interacts with transcriptional activators such as the androgen receptor to promote transcription. Disruption of TAF1 causes dystonia 3 (X-linked torsion dystonia) and decreased apoptosis. This protein product contains the first bromodomain of TAF1.

7660 | TAF1 bromodomain 1 1371-1496 aa GST-tagged Human recombinant DataSheet

Biomolecule/Target: TAF1

Synonyms: TAF1 bromodomain 1 (1371-1496 aa) (GST-tagged), Human recombinant

Alternates names: TATA Box Binding Protein (TBP)-Associated Factor; KAT4; DYT3; CCGS; NSCL2; XDP; TAF1 RNA Polymerase II; TAF2A; TAFII250; BA2R; CCG1

Taglines: A component of transcription factor IID

NCBI Gene ID #: 7043

NCBI Gene Symbol: TGFB3

Gene Source: Human

Accession #: P10600

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: ’s recombinant human TGF-3 is fully biologically active when compared to standard. The ED determined by cytotoxicity assay using the world health organization standard 98/608 as a direct comparison is <0.05 ng/ml, corresponding to a specific activity of >2 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 41.7 kDa (1371-1496 aa + NT GST Tag)

Concentration: N/A

Appearance: Liquid

Physical form description: 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5 mM -mercaptoethanol and 5% glycerol.

Reconstitution Instructions: This protein solution should be stored at 4°C, stable for at least 3 months. For long term storage, it is recommended to add a carrier protein (0.1% HAS or BSA).

Amino acid sequence: N/A