LBD
TAP2 Antibody (N-Term) [APR33594G]
- SKU:
- LBD-APR33594G
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Description
TAP2 Antibody (N-Term) [APR33594G] | Gentaur UK, US & Europe Distribution
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This TAP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 100-134 amino acids from the human region of human TAP2.
Cellular Localisation: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=The transmembrane segments seem to form a pore in the membrane
Molecular Weight: 75664
Clone: Polyclonal
Gene Name: TAP2 {ECO:0000303|PubMed:10605026, ECO:0000312|HGNC:HGNC:44}
Gene ID: 6891
Function: ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (PubMed:25656091, PubMed:25377891) . Uses the chemical energy of ATP to export peptides against the concentration gradient (PubMed:25377891) . During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (PubMed:25377891, PubMed:25656091, PubMed:11274390) . Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect (PubMed:7500034, PubMed:9256420, PubMed:11274390) . As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (PubMed:26611325, PubMed:1538751, PubMed:25377891) .
Summary: N/A
Form: N/A
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB, FC
Dilution: FC--1:25 WB--1:2000
Synonyms: Antigen peptide transporter 2, APT2, ATP-binding cassette sub-family B member 3, Peptide supply factor 2, Peptide transporter PSF2, PSF-2, Peptide transporter TAP2, Peptide transporter involved in antigen processing 2, Really interesting new gene 11 protein, TAP2, ABCB3, PSF2, RING11, Y1