Description
Tarc Antibody (biotin) | XP-5279Bt | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTARC (human TARC) .
Research Area: Chemokines & Cytokines, Immunology, Antibody Pairs
Tested Application: E, WB
Application: ELISA:
Sandwich:
To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TARC (XP-5279) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTARC.
Western Blot:
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti-hTARC specific antibody was purified by affinity chromatography and then biotinylated.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Biotin
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: Tarc antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: TARC, ABCD-2, SCYA17, A-152E5.3, TARC, C-C motif chemokine 17, CC chemokine TARC,
User Note: Centrifuge vial prior to opening.
BACKGROUND: Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TARC displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. TARC binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells