Description
TCF3 Antibody | 28-832 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Dog
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF3.
Research Area: Transcription, Cancer
Tested Application: E, WB
Application: TCF3 antibody can be used for detection of TCF3 by ELISA at 1:1562500. TCF3 antibody can be used for detection of TCF3 by western blot at 0.31 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 68 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein A chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: TCF3, E2A, E47, ITF1, VDIR, TCF-3, bHLHb21
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TCF3 contains 1 basic helix-loop-helix (bHLH) domain. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. TCF3 binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.