TCP-1 Antibody | 45-158

TCP-1 Antibody | 45-158 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: N/A

Immunogen: The immunogen for this antibody is: C-SYEDAVHSGALND

Research Area: Neuroscience

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3kDa according to NP_110379.2) . Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognise both reported isoforms.

Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1

User Note: N/A

BACKGROUND: N/A