TECK (CCL25), human recombinant | 7214

TECK is a CC chemokine, specifically expressed by thymic stromal cells, and signals through the CCR9 receptor. TECK is chemotactic towards activated macrophages, thymocytes and dendritic cells. Recombinant human TECK is a 14.2 kDa protein containing 127 amino acid residues, including the four conserved cysteine residues present in CC chemokines.

7214 | TECK CCL25 human recombinant DataSheet

Biomolecule/Target: CCL25

Synonyms: Thymus Expressed Chemokine, CCL25

Alternates names: CPG15, NRN1

Taglines: Chemotactic towards activated macrophages, thymocytes and dendritic cells

NCBI Gene ID #: 51299

NCBI Gene Symbol: NRN

Gene Source: Human

Accession #: Q9NPD7

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by a cell proliferation using rat C6 cells. The ED50 for this effect is 20.0-30.0 ng/ml.

Biological activity: Determined by a cell proliferation using rat C6 cells. The ED50 for this effect is 20.0-30.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 9.72 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN