TFAM Antibody (C-term) [APR10397G]

TFAM Antibody (C-term) [APR10397G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM.

Cellular Localisation: Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid

Molecular Weight: 29097

Clone: Polyclonal

Gene Name: TFAM (HGNC:11741)

Gene ID: 7019

Function: Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942) . Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603) . In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300) . Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172) . Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172) . Is able to unwind DNA (PubMed:22037172) . Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790) . Required for maintenance of normal levels of mitochondrial DNA (PubMed:22841477, PubMed:19304746) . May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, FC, IF, WB

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50 IF--1:10~50

Synonyms: Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM, TCF6, TCF6L2