TFF-3, human recombinant | 4746

Proteins of the TFF family are characterized by having at least 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, through an EGFR-dependent pathway.TFF-3 is normally expressed in hepatocellular carcinoma and its overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved disulfide bonds and has MW = 13.2 kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

4746 | TFF-3 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Trefoil factor 3

Alternates names: Serpin I1, Protease inhibitor 12

Taglines: A protein which protects and stabilizes the gastric mucus layer

NCBI Gene ID #: 5274

NCBI Gene Symbol: SERPINI1

Gene Source: Human

Accession #: Q99574

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 96%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 g/ml.

Biological activity: Determined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 44.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate pH 7.8 and 50 mM NaCl

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL