TGF-1, human recombinant (E. Coli) | 7843

Transforming growth factor-betas (TGF-s) mediate many cell-cell interactions that occur during embryonic development. Three TGFs have been identified in mammals. TGF-Beta 1, TGF-Beta 2 and TGF-Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. TGF-beta 1 is involved in hematopoiesis and endothelial differentiation.

7843 | TGF-1 human recombinant E. Coli DataSheet

Biomolecule/Target: TGF-1

Synonyms: Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Alternates names: Platelet-Derived Growth Factor-CC

Taglines: 95% Pure Human recombinant TGF-1 that controls proliferation, cellular differentiation, & other functions in most cells

NCBI Gene ID #: 56034

NCBI Gene Symbol: PDGFC

Gene Source: Human

Accession #: Q9NRA1

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.

Biological activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium citrate, pH 3.0

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG