TGF-alpha, human recombinant | 4339

Transforming Growth Factor- (TGF-) stimulates the proliferation of a wide range of epidermal and epithelial cells. TGF- has been shown to play a role in the autocrine growth of certain transformed cells. Human TGF- is a 5.5 kDa protein containing 50 amino acid residues.

4339 | TGF-alpha human recombinant DataSheet

Biomolecule/Target: TGF-alpha

Synonyms: Transforming Growth Factor-, Sacroma growth factor, TGF-type I, ETGF

Alternates names: Serpin I1, Protease inhibitor 12

Taglines: A growth factor upregulated in some cancers

NCBI Gene ID #: 5274

NCBI Gene Symbol: SERPINI1

Gene Source: Human

Accession #: Q99574

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 96%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 g/ml.

Biological activity: Determined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 44.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate pH 7.8 and 50 mM NaCl

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL