THEM4 Antibody | 5227

THEM4 Antibody | 5227 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4.
The immunogen is located within amino acids 60 - 110 of THEM4.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: Cat. No. 10-201 - Human Liver Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: THEM4 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: THEM4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: THEM4 Antibody: CTMP, CTMP, Acyl-coenzyme A thioesterase THEM4, Carboxyl-terminal modulator protein, Acyl-CoA thioesterase THEM4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.