Thioredoxin Antibody| F40029-0.4ML/RQ5327

Thioredoxin Antibody| F40029-0.4ML/RQ5327 | Gentaur UK, US & Europe Distribution

Background: Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1.Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

Format: Purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: N/A

Application: WB, ELISA

Application Details: Western blot: 1:1000

Application Note: Titration of the Thioredoxin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This Thioredoxin antibody is available for research use only.

Purity: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this Thioredoxin antibody.

Storage: Aliquot the Thioredoxin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A