Thrombomodulin Antibody [THBD/1782] | 33-688

Thrombomodulin Antibody [THBD/1782] | 33-688 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.

Research Area: Stem Cell

Tested Application: WB, IHC-P, Flow, IF, E

Application: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 0.5-1 ug/ml
IHC (FFPE) : 1-2 ug/ml for 30 min at RT
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: THBD/1782

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma) . Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.